Accession Number : ADA306911

Title :   Molecular Biological Approaches to Studying Cyanide Detoxification by Rhodanese.

Descriptive Note : Final rept. 16 May 94-15 Dec 95,

Corporate Author : SOUTHWEST FOUNDATION FOR BIOMEDICAL RESEARCH SAN ANTONIO TX

Personal Author(s) : Dooley, Thomas P.

PDF Url : ADA306911

Report Date : JAN 1995

Pagination or Media Count : 20

Abstract : Rhodanese (thiosulfate sulfurtransferase) is expressed as high levels in liver and involved in the detoxification of cyanide. The full-length cDNA corresponding to the mouse rhodanese gene (Tst), which is located on chromosome 15, was cloned by PCR amplification of a liver cDNA library and subjected to DNA sequencing. Alignment of the rhodanese cDNA sequences from mouse and rat, which we previously cloned (Biochem. J. 275:227-231 revealed 97.3 percent identity at the protein level and 94.6 percent identity at the DNA leve When the mouse and rat cDNAs were expressed under the control of IPTG-inducible promoters in E. Coli, the cell extracts exhibited cyanide-metabolizing activity, indicating that both genes encode functional rhodanese molecules. The results of the study were published by Dooley al. in Biochemical and Biophysical Research Communications 216:1101-1109 (1995).

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *GENES, *CLONES, *CYANIDES, *THIOCYANATES, BIOLOGY, RATS, MOLECULES, PROTEINS, CHROMOSOMES, ESCHERICHIA COLI, MICE, LIVER, CELLS(BIOLOGY), DETOXIFICATION.

Subject Categories : Toxicology
      Biochemistry
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE