Accession Number : ADA307114

Title :   X-Ray Crystallographic Studies on Acetylcholinesterase and on its Interaction with Anticholinesterase Agents.

Descriptive Note : Final rept. 30 Apr 93-30 Sep 95,

Corporate Author : WEIZMANN INST OF SCIENCE REHOVOT (ISRAEL)

Personal Author(s) : Silman, Israel ; Sussman, Joel

PDF Url : ADA307114

Report Date : DEC 1995

Pagination or Media Count : 73

Abstract : The objective of this project was to solve, for the first time, by X-ray crystallography, the three-dimensional structure of acetylcholinesterase (AChE) and to learn from this structure the reaction mechanism, inhibition modes and other structural and functional properties of the enzyme. The principal objectives of the current project included: (1) Attempts to obtain crystals of cholinesterases other than T. californica AChE, in particular of AChE of human origin. (2) Collection of improved data sets, viz. at higher resolution than previously, for crystals of Torpedo AChE. (3) Determination of complexes of Torpedo AChE with various ligands, including orgamophosphates. In the this project, we have made progress towards all three objectives, as well as in additional areas related to the long-term aims of our research program

Descriptors :   *CRYSTALS, *X RAYS, *ACETYLCHOLINESTERASE, *CHOLINESTERASE, *CRYSTALLOGRAPHY, DATA BASES, TORPEDOES, HUMANS, STRUCTURAL PROPERTIES, ENZYMES, STRUCTURES, HIGH RESOLUTION, THREE DIMENSIONAL, RESPONSE, LIGANDS, FUNCTIONAL ANALYSIS, INHIBITION, CHOLINESTERASE INHIBITORS.

Subject Categories : Biochemistry
      Radiobiology
      Crystallography

Distribution Statement : APPROVED FOR PUBLIC RELEASE