Accession Number : ADA307152

Title :   Calcium Carbonate Mineralization.

Descriptive Note : Final rept. 1 Feb 92-30 Sep 94,

Corporate Author : DUKE UNIV DURHAM NC OFFICE OF SPONSORED PROGRAMS

Personal Author(s) : Wilbur, Karl M. ; LeFurgey, Ann

PDF Url : ADA307152

Report Date : 20 JUL 1995

Pagination or Media Count : 12

Abstract : The objectives of the present study were to determine the mechanisms of ion transport across mineralizing epithelia of barnacles, in particular to quantify the effects of altered protein structure on crystal form and arrangement and the effects of the molting hormone 20-OH ecdysone on calcium carbonate deposition rate and growth increment formation. The main technical focus was to develop new methods for monitoring and measuring the mineralization process. The studies of biomineralization by the principal investigator undertaken in the early 1950s demonstrated for the first time that (45)Ca could be used to measure the rate of mineral deposition in shells of marine molluscs. These studies conducted during 1992-1994 demonstrate, for the first time, that elemental probes, such as strontium and manganese, can substitute for calcium in mineral formation. Semi-quantitative electron probe x-ray images of Sr and Mn revealed that their incorporation is cyclical, directly related to addition of growth increments, and proportional to the amount of element in the sea water environment. This use of elemental probes, coupled with the development of a functional in vitro mineralizing epithelial preparation, makes possible a new approach to invertebrate mineralization studies.

Descriptors :   *EPITHELIUM, *CALCIUM COMPOUNDS, *ION EXCHANGE, *CARBONATES, *MINERALIZATION, PROBES, HORMONES, PREPARATION, GROWTH(GENERAL), PROTEINS, CRYSTALS, X RAYS, IN VITRO ANALYSIS, DEPOSITION, ELECTRONS, IMAGES, TRANSPORT, INVERTEBRATES, SEA WATER, OCEAN ENVIRONMENTS, STRONTIUM, MINERALS, CHEMICAL ELEMENTS, MANGANESE, MOLLUSCA.

Subject Categories : Inorganic Chemistry
      Physical Chemistry
      Biological Oceanography
      Geology, Geochemistry and Mineralogy

Distribution Statement : APPROVED FOR PUBLIC RELEASE