Accession Number : ADA307278

Title :   Evaluation of Dried Storage of Platelets for Transfusion with Emphasis on Physiologic Integrity and Hemostatic Functionality.

Descriptive Note : Final rept. 1 Feb 92-30 Jun 95,

Corporate Author : EAST CAROLINA UNIV SCHOOL OF MEDICINE GREENVILLE NC

Personal Author(s) : Bode, Arthur P. ; Read, Marjorie S.

PDF Url : ADA307278

Report Date : 24 JAN 1996

Pagination or Media Count : 40

Abstract : This project was a follow-on to the original phase of development of freeze-dried platelets for transfusion under prior ONR grant N00014-89-J-1712. During the 3 1/2 year performance period, we met our goals of evaluation of a standard method of stabilizing and lyophilizing blood platelets from human or animal sources in several in vitro and in vivo test systems. The mechanism of platelet adhesion was found to be intact in our lyophilized platelet preparations, and evidence of residual metabolic capability was demonstrated. Two different long-term storage studies (up to 1 year) were carried out at various temperatures, which showed some instability at 22 deg C but none at 4 deg C or -70 deg C. Scale-up of production with sterile technique was begun in our research labs but transferred to Armour Pharmaceutical Corp, as they entered the ATD program. The in vivo hemostatic efficacy of rehydrated platelet preparations was demonstrated in several animal models involving normalization of a prolonged bleeding time in thrombocytopenic rats or rabbits, and in von Willebrand's disease or normal dogs. Labelled rehydrated platelets were found histologically in wound sites in infused dogs. These findings show the potential utility of these preparations in transfusion medicine.

Descriptors :   *DRYING, *BLOOD TRANSFUSION, *HEMORRHAGE, *BLOOD PLATELETS, *LYOPHILIZATION, TEST AND EVALUATION, ARMOR, SOURCES, PRODUCTION, PREPARATION, MODELS, SITES, ADHESION, LONG RANGE(TIME), METABOLISM, FREEZE DRYING, IN VITRO ANALYSIS, TIME, MEDICINE, RESIDUALS, SCALING FACTOR, STANDARDIZATION, ANIMALS, PHYSIOLOGY, WOUNDS AND INJURIES, TRANSFER, STORAGE, IN VIVO ANALYSIS, DOGS, RABBITS, STERILIZATION, BLOOD COAGULATION.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE