Accession Number : ADA307279

Title :   Precllnical Investigations of Lyophilized Platelet Preparations.

Descriptive Note : Triannual rept. no. 2, 1 Jan-30 Apr 95,

Corporate Author : EAST CAROLINA UNIV SCHOOL OF MEDICINE GREENVILLE NC

Personal Author(s) : Bode, Arthur P. ; Read, Marjorie S.

PDF Url : ADA307279

Report Date : 05 JUL 1995

Pagination or Media Count : 24

Abstract : During this period of activity, we published our first comprehensive journal article from results in this project: see Proceedings of the National Academy of Sciences 92:397-401, 1995 (attached). A no-cost extension of the project was approved to extend activities to 31 Aug 96. At the ECU performance site, four trials of hemostatic efficacy of our rehydrated lyophilized platelet preparations were carried out in live pigs according to the Letterman Army Institute animal model of hemorrhagic shock. The results were quite variable and showed the need to adapt this model to emphasize the role of platelets and control other physiologic parameters related to the effects of hypovolemic shock. Attempts in vitro to load rehydrated platelets with calcium ion flux indicator dyes were finally successful, and studies are now underway to measure the responsiveness of these platelet preparations to thrombin and other agonists that rely on Ca2+ for signal transduction. Experiments conducted at UNC showed serotonin uptake and release by rehydrated platelets and surface-related changes in activation. It will be important to continue to define the extent of activation and its control in our rehydrated lyophilized platelet preparations.

Descriptors :   *CLINICAL MEDICINE, *BLOOD PLATELETS, *LYOPHILIZATION, *BLOOD COAGULATION, ACTIVATION, PREPARATION, MODELS, PARAMETERS, SITES, IN VITRO ANALYSIS, COSTS, SIGNALS, TRANSDUCERS, ANIMALS, PHYSIOLOGY, SEROTONIN, WOUNDS AND INJURIES, HEMORRHAGIC SHOCK, TRAUMATIC SHOCK, SWINE, THROMBIN.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE