Accession Number : ADA307322

Title :   Construction of a Recombinant Viral Vector Containing Part of the Nucleocapsid Protein Gene of Newcastle Disease Virus,

Corporate Author : DEFENCE RESEARCH ESTABLISHMENT SUFFIELD RALSTON (ALBERTA)

Personal Author(s) : Bader, Douglas E.

PDF Url : ADA307322

Report Date : SEP 1995

Pagination or Media Count : 36

Abstract : This report describes the procedures used to clone a 673 base pair gene fragment of the major nucleocapsid protein gene of Newcastle disease virus into a viral vector molecule for the purpose of maintaining a stable, long-term, renewable source of this target sequence for gene probe studies. The gene fragment was prepared by reverse-transcription polymerase chain reaction of Newcastle disease virus RNA and was cloned into the viral DNA vector Ml3mp18 RF to produce a recombinant DNA molecule. The cloned fragment was shown to be present in the recombinant clones based on (i) clonal selection on indicator plates; (ii) restriction enzyme analysis; (iii) gene probe analysis and (iv) nested PCR amplification.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *GENES, *CLONES, *PAIR PRODUCTION, *RIBONUCLEIC ACIDS, *CHAIN REACTIONS, *NEWCASTLE DISEASE VIRUS, PROBES, MOLECULES, ENZYMES, PROTEINS, TARGETS, SEQUENCES, PLATES, REVERSIBLE, VECTOR ANALYSIS, PARTS, GENETIC ENGINEERING, VIRUSES, INDICATORS, SELECTION.

Subject Categories : Microbiology
      Biochemistry
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE