Accession Number : ADA307406

Title :   Molecular Probes and Bioluminescent Reporters in Ecological Optimization of Biodegradation.

Descriptive Note : Final rept. 1 Jun 92-31 May 95,

Corporate Author : TENNESSEE UNIV KNOXVILLE CENTER FOR ENVIRONMENTAL BIOTECHNOLOGY

Personal Author(s) : Sayler, G. S.

PDF Url : ADA307406

Report Date : 31 MAY 1995

Pagination or Media Count : 4

Abstract : DNA probe and bioluminescent sensor technology is being used to assess the bio-availability of sorbed or immiscible-phase toluene and polycyclic aromatic hydro-carbons (PAR) in particulate media. Construction of improved bioluminescent reporter strains for PAH and toluene (also trichloroethylene) degradation is ongoing. For PAR degradation, the approach involves incorporation of a transposon containing the lower naphthalene pathway promoter fused to the lux genes (nah-lux) into the bacterial chromosome. One of the two transposons (Tn5-based transposon) appears to be successful in forming the fusion product and incorporating into the Psuedomonas genomes. Work is ongoing. For toluene biodegradation, the approach involves a bacterial strain containing a plasmid-encoded tod-lux gene fusion. The strain produces light when the inducer, toluene, signals an increased production of the catabolizing enzyme, toluene diooxygenase. Finally, a related project involves the - development of a combined method to extract and analyze both DNA and lipids from the same environmental sample in order to maximize the informational content of a single sample with respect to biomass content, community structure and the physological status of microrganisms.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *TRICHLOROETHYLENE, *TOLUENES, *BIOLUMINESCENCE, PROBES, OPTIMIZATION, DETECTORS, COMMUNITIES, DEGRADATION, MOLECULES, BACTERIA, ENZYMES, LIPIDS, CHROMOSOMES, STRAINS(BIOLOGY), AROMATIC HYDROCARBONS, PARTICULATES, MEDIA, GENES, BIODEGRADATION, ECOLOGY, MICROORGANISMS, BIOMASS CONVERSION, NAPHTHALENES.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE