Accession Number : ADA308053

Title :   Analysis of the Regulation of Expression of Transforming Growth Factor-Beta in Human Breast Cancer Cells.

Descriptive Note : Annual rept. 1 Oct 94-30 Sep 95,

Corporate Author : DARTMOUTH COLL HANOVER NH

Personal Author(s) : Arrick, Bradley A.

PDF Url : ADA308053

Report Date : OCT 1995

Pagination or Media Count : 8

Abstract : This grant entails two objectives. These objectives derived from prior work by ourselves and others which have identified TGF-B as an important cytokine in the biology of breast cancer. For established tumors, overexpression of TGF-B may result in increased in vivo tumor growth and metastatic spread. The first objective involves the analysis of resected breast cancer specimens, with the ultimate goal of determining the mechanism(s) by which TGF-B could be overexpressed by some tumors. There are as yet no definitive data or conclusions from this aspect of the work. The second objective of the grant was to identify the molecular determinants of promoter usage for TGF-B3 in breast cancer cells. These studies stem from our prior work in which we have characterized an alternative TGF-B3 promoter uniquely functional in breast cancer cells. We have made some progress in designing promoter constructs which yield authentic promoter function upon transfection into breast cancer cells. The Statement of Work did not call for any conclusions by this point in the grant, and none are available.

Descriptors :   *CELLS(BIOLOGY), *GROWTH(PHYSIOLOGY), *BREAST CANCER, CONTROL, BIOLOGY, HUMANS, NEOPLASMS, GENES, IMMUNITY, IN VIVO ANALYSIS.

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE