Accession Number : ADA308301

Title :   Genetic Toxicity Evaluation of 1,1,1,2,3,3,3- Heptatfluoropropane. Volume 1. Results of Salmonella Typhimurium Histidine Reversion Assay (Ames Assay).

Descriptive Note : Final rept. Mar-Dec 94,

Corporate Author : MANTECH ENVIRONMENTAL TECHNOLOGY INC RESEARCH TRIANGLE PARK NC TOXIC HAZARD RE SEARCH UNIT

Personal Author(s) : Mitchell, A. D.

PDF Url : ADA308301

Report Date : JAN 1995

Pagination or Media Count : 23

Abstract : Under subcontract to ManTech Environmental Technology, Incorporated, Uenesys Research, Incorporated tested 1,1,12,3,3,3- heptafluoropropane (HFC-227ea) using Billups-Rothenberg exposure chambers for the exposure chamber modification of the Salmonella typhimurium histidine (his) reversion mutagenesis system (the Ames test), a microbial assay that measures his his+ reversion induced by chemicals that cause base changes or frameshift mutations i the genome of this organism. Testing was conducted using five Salmonella strains, with and without metabolic activation. HFC-227ea was tested in a preliminary test and in a mutagenesis assay. HFC-227ea was tested to toxic levels in the mutagenesis assay, but a sufficient number of nontoxic concentrations were tested to determine if HFC-227ea were capable of inducing a dose-related mutagenic response, and the positive control responses were consistent with historical data from the laboratory, and no evidence of a mutagenic response was obtained in any strain without or with activation. Therefore, HFC-227ea was negative in the Salmonella typhimurium histidine reversion mutagenesis test in the presence and absence of metabolic activation.

Descriptors :   *TOXICITY, *GENETICS, *MICROORGANISMS, *SALMONELLA TYPHIMURIUM, TEST AND EVALUATION, CONTROL, ACTIVATION, ENVIRONMENTS, EXPOSURE(GENERAL), MODIFICATION, METABOLISM, MUTATIONS, RESPONSE, HISTORY, LEVEL(QUANTITY), EXPOSURE(PHYSIOLOGY), RESPONSE(BIOLOGY), MUTAGENS, ASSAYING, CHAMBERS, HISTIDINE.

Subject Categories : Genetic Engineering and Molecular Biology
      Toxicology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE