Accession Number : ADA308975

Title :   Molecular Biology of Anaerobic Aromatic Biodegradation.

Descriptive Note : Final rept. 15 Jul 92-15 Jul 96,

Corporate Author : IOWA UNIV IOWA CITY DEPT OF MICROBIOLOGY

Personal Author(s) : Harwood, Caroline S.

PDF Url : ADA308975

Report Date : 06 APR 1996

Pagination or Media Count : 6

Abstract : The molecular basis for the anaerobic degradation of benzoate and 4-hydroxybenzoate was investigated using the bacterium Rhodopseudomonas palustris as a model. These aromatic acids are intermediates in the degradation of structurally diverse aromatic compounds, includilng environmental pollutants, by many metabolic types of anaerobic bacteria. Benzoate is the starting compound for a major central pathway of anaerobic benzene ring reduction and cleavage. This appears to be the major route required for complete degradation of toxic aromatic compounds under anaerobic conditions. There is still uncertainty, however, about the sequence of intermediates formed in the benzoate pathway and very little is known about the enzymology and genetics of the pathway. A major thrust of the work involved cloning benzoate degradation and regulatory genes and assigning functions to these genes based on nucleotide sequencing and physiological analyses of strains carrying defined mutations. Other studies included purification and characterization of the benzoate pathway ring cleavage enzyme (2-ketocyclohexanecarboxyl-CoA hydrolase).

Descriptors :   *ENZYMES, *ANAEROBIC BACTERIA, *BENZOATES, UNCERTAINTY, BENZENE, ENVIRONMENTS, DEGRADATION, TOXICITY, MUTATIONS, REDUCTION, PURIFICATION, XYLENES, MOLECULAR BIOLOGY, GENES, CLONES, POLLUTANTS, AROMATIC COMPOUNDS, RINGS, GENETIC ENGINEERING, BIODEGRADATION, THRUST, CLEAVAGE, CARBOXYLIC ACIDS, ACIDS, NUCLEOTIDES, ANAEROBIC PROCESSES.

Subject Categories : Microbiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE