Accession Number : ADA309744

Title :   Two-Dimensional Protein Pattern Recognition in Chemical Toxicity.

Descriptive Note : Final technical rept. 1 Apr 93-31 Mar 96,

Corporate Author : INDIANA UNIV-PURDUE UNIV AT COLUMBUS DEPT OF BIOLOGY

Personal Author(s) : Witzmann, Frank A.

PDF Url : ADA309744

Report Date : 15 MAY 1996

Pagination or Media Count : 40

Abstract : The mechanisms underlying the effects of chemical toxicity such as cell damage, necrosis, and death, as well as repair, involve changes in the abundance and qualitative character of specific proteins in target tissues and relevant in vitro systems. One way to detect such toxic effects, to determine the mechanism(s) involved in a specific effect among a broad range of cellular mechanisms, and to predict the effects of structurally similar compounds, would involve a system that enables simultaneous analysis of greater than 1000 target cell proteins with respect to their response to chemical intoxication. Recognizable patterns of protein alteration so documented would serve as biomarkers of injury or repair, or both. This final technical report documents the progress made during the research period regarding the application of large-scale two-dimensional electrophoresis (2DE) and image analysis to Air Force Toxicologic interests and describes, in particular, the development of prototype tissue 2DE protein databases; multi-species, multi-tissue, and serum 2DE protein map development; and stress protein biomarker development in rodent and human liver samples.

Descriptors :   *CHEMICALS, *TOXICITY, *PROTEINS, *PATTERN RECOGNITION, *CELLS(BIOLOGY), *INTOXICATION, STRESSES, IMAGE PROCESSING, AIR FORCE, TISSUES(BIOLOGY), DAMAGE, HUMANS, TWO DIMENSIONAL, IN VITRO ANALYSIS, SAMPLING, CHEMICAL AGENTS, PATTERNS, WOUNDS AND INJURIES, MARKERS, SYNCHRONISM, LIVER, DEATH, ELECTROPHORESIS, CYTOLOGY, TRACER STUDIES.

Subject Categories : Biochemistry
      Toxicology
      Cybernetics

Distribution Statement : APPROVED FOR PUBLIC RELEASE