Accession Number : ADA310710

Title :   Cholinesterase Structure: Identification of Residues and Domains Affecting Organophosphate Inhibition and Catalysis.

Descriptive Note : Annual rept. 6 Mar 95-5 Mar 96,

Corporate Author : CALIFORNIA UNIV SAN DIEGO LA JOLLA

Personal Author(s) : Taylor, Palmer W.

PDF Url : ADA310710

Report Date : APR 1996

Pagination or Media Count : 124

Abstract : In the initial year of the grant, we have made excellent progress in several arenas: (1) A crystal structure of a mouse acetylcholinesterase-fasciculin 2 complex has been solved. (2) Studies with enantiomeric organophosphates have yielded vital information on their binding orientation in the ground and transition states. (3) Studies in oxime reactivation of inhibited cholinesterase have uncovered the basis for enhanced reactivity of HI-6 compared to 2-PAM. (4) The interactions of fasciculin 2 with acetylcholinesterase have been studied by kinetic and site-specific mutagenesis methods.

Descriptors :   *CRYSTAL STRUCTURE, *CATALYSIS, *ACETYLCHOLINESTERASE, *CHOLINESTERASE INHIBITORS, ACTIVATION, ORIENTATION(DIRECTION), X RAYS, MUTATIONS, IDENTIFICATION, TRANSITIONS, KINETICS, INHIBITION, CHOLINESTERASE, RESIDUES, ORGANOPHOSPHATES, OXIMES.

Subject Categories : Biochemistry
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE