Accession Number : ADA311176

Title :   In Vivo Studies of Hematopoietic Growth Factors for Use in Military Personnel.

Descriptive Note : Interim progress rept. 1 Jun-30 Sep 95,

Corporate Author : NEW ENGLAND DEACONESS HOSPITAL BOSTON MA CANCER RESEARCH INST

Personal Author(s) : Sytkowski, Arthur J.

PDF Url : ADA311176

Report Date : 25 OCT 1995

Pagination or Media Count : 8

Abstract : The secondary structures and thermal denaturation/renaturation of recombinant human erythropoietins were compared using UV circular dichroism. Marked differences were observed between proteins expressed by different host cell lines, presumably due to dissimilar glycosylation patterns. A pharmacokinetic study of monomeric erythropoietin carried out in baboons demonstrated a very short plasma half-life of three hours. The proper formulation of the protein for these studies was established. Work on erythropoietin +dimers progressed, but high yields in each reaction were different to achieve. Knowledge of the differing glycosylation patterns will help improve these yields. Work on erythroid colony stimulation factor was resumed, and rodent bone marrow cultures were established.

Descriptors :   *MILITARY PERSONNEL, *IN VIVO ANALYSIS, *HEMATOPOIETIC SYSTEM, *GROWTH(PHYSIOLOGY), *HEMATOPOIESIS, *ERYTHROPOIETINS, ULTRAVIOLET RADIATION, HUMANS, PLASMAS(PHYSICS), PROTEINS, FORMULATIONS, STRUCTURES, PHARMACOKINETICS, RODENTS, CULTURES(BIOLOGY), CIRCULAR, DICHROISM, BONE MARROW, DIMERS, HOSTS(BIOLOGY), BABOONS, HALF LIFE.

Subject Categories : Personnel Management and Labor Relations
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE