Accession Number : ADA311477

Title :   Development and Comparison of Different Peptide IgG Conjugates for the Treatment of Gram-Negative Sepsis.

Descriptive Note : Progress rept. 7 Mar-22 Jun 95,

Corporate Author : MASSACHUSETTS GENERAL HOSPITAL BOSTON

Personal Author(s) : Warren, H. S.

PDF Url : ADA311477

Report Date : 22 JUN 1995

Pagination or Media Count : 23

Abstract : Work over the trimester focused on defining the best LPS binding protein (LBP) peptide sequence, generating peptide-IgO conjugates with this sequence, and assessing the functional ability of these conjugates in buffer and serum. Preliminary work suggested that these, and similar peptides composed of CAPl8 and BPI peptides, lost activity in serum compared to buffer. We compared the bactericidal activity of the LBP peptides in serum and buffer, and assessed the ability of conjugates made with these peptides to bind LPS in serum and buffer. We also evaluated the effect of a terminal cysteine on the peptide on peptide activity. We found that that killing was reduced in serum compared to buffer, and that the ability of the LBP peptide-IgG conjugates to bind radiolabeled LPS was reduced in serum compared to buffer. However, the activity of the peptides was dramatically increased with a terminal cysteine. Experiments using 14C-peptide confirmed previous estimates that constructs contained 3.9 and 9.5 peptides/IgG.

Descriptors :   *PEPTIDES, *GRAM NEGATIVE BACTERIA, *SEPSIS, BACTERIA, IN VITRO ANALYSIS, SEQUENCES, CHEMICAL BONDS, BLOOD SERUM, CYSTEINE, ENDOTOXINS, BLOOD PLASMA, GERMICIDES.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE