Accession Number : ADA311764

Title :   Autoradiographic Localization of (125I)-Ricin in Lungs and Trachea of Mice Following an Aerosol Inhalation Exposure.

Descriptive Note : Technical rept.,

Corporate Author : ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Personal Author(s) : Doebler, Jeffrey A. ; Moeller, Robert B. ; Wiltshire, Norman D. ; Mayer, Thomas W. ; Estep, James E.

PDF Url : ADA311764

Report Date : JUN 1996

Pagination or Media Count : 10

Abstract : Studies were conducted to examine the light microscopic distribution of (1251)-ricin within the lungs and corresponding lung pathology in mice following single, nose-only, aerosol inhalation exposures. Groups of 4-8 mice were euthanatized 15 minutes and 1, 4, 6, 8, 12, and 24 hours post-exposure. At 15 minutes post-exposure, abundant radiolabel was present within alveolar spaces. At all later times, however, (125l)-ricin was observed primarily within the lumina of bronchioles and larger passageways. Cellular targets for ricin within the lungs could not be identified using the techniques employed; it is therefore recommended that ultrastructural autoradiography be used to determine cellular ricin binding targets. The primary pathological abnormality detected was multifocal epithelial necrosis of the secondary bronchioles.

Descriptors :   *LUNG, *RICIN, *INHALATION, *AUTORADIOGRAPHY, *TRACHEA, EPITHELIUM, AEROSOLS, DISTRIBUTION, CELLS, LIGHT, MICROSCOPY, PATHOLOGY, EXPOSURE(PHYSIOLOGY), ABNORMALITIES, NECROSIS, ALVEOLI.

Subject Categories : Anatomy and Physiology
      Microbiology
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE