Accession Number : ADA312072

Title :   Neuropharmacological Characterization of Botulinum Neurotoxin,

Descriptive Note : Rept. for 1 Apr 95-31 Mar 96,

Corporate Author : JEFFERSON MEDICAL COLL PHILADELPHIA PA

Personal Author(s) : Simpson, Lance L.

PDF Url : ADA312072

Report Date : APR 1996

Pagination or Media Count : 54

Abstract : Experiments have been done to clarify the nature of botulinum toxin binding. This work is an essential prelude to efforts to identify drugs that can antagonize toxin action by blocking toxin association with cell surface receptors. During the first part of the work, experiments were done: (1) to identify human tissue preparations that can be used to study electrophysiology, ligand binding, and certain aspects of molecular biology, and (2) to examine botulinum toxin action on these tissues. This work resulted in the findings that the human pyramidalis muscle can be used as a model to study neuromuscular transmission, and cerebellar synaptosomes can be used as models to study CNS function. During the second part of the work, the process of characterizing toxin receptors began. This work resulted in the finding that: (1) toxin receptors are found in most parts of the nervous system, but distribution is very uneven, and (2) molecular biology techniques can be used to transfer functioning receptors from the nervous system to a model translation system, i.e., Xenopus oocytes.

Descriptors :   *DRUGS, *TOXINS AND ANTITOXINS, *BACTERIAL TOXINS, *CLOSTRIDIUM BOTULINUM, FUNCTIONS, TISSUES(BIOLOGY), PREPARATION, MODELS, LIGANDS, NERVOUS SYSTEM, MOLECULAR BIOLOGY, TRANSLATIONS, HUMAN BODY, BLOCKING, CENTRAL NERVOUS SYSTEM, ELECTROPHYSIOLOGY, NEUROMUSCULAR TRANSMISSION.

Subject Categories : Microbiology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE