Accession Number : ADA313737

Title :   Identification of Nucleic Acid Sequences and Chemical Markers for Taxonomic Characterization of Bacteria.

Descriptive Note : Final rept. Jun 92-Jun 96,

Corporate Author : SOUTH CAROLINA UNIV COLUMBIA DEPT OF MICROBIOLOGY AND IMMUNOLOGY

Personal Author(s) : Fox, Alvin

PDF Url : ADA313737

Report Date : 14 AUG 1996

Pagination or Media Count : 7

Abstract : Our biodetection research is primarily concerned with two inter-related issues (1) development of novel schemes for improving chemotaxonomic characterization of microbial pathogens and (2) mass spectrometry-based methods for trace detection of chemical markers for key agents in complex environmental matrices. $180,000 in direct + indirect costs were provided over the period June 1992-1995. This contract was extended with additional funds of $49,020 from ERDEC through June 1995- June 1996. Development of chemotaxonomic schemes for correct identification of species of bacteria relevant to the biodetection program is essential. The research employed a systematic search for new chemical markers employing state-of-the-art chemical and molecular approaches. The feasibility of detecting markers for bacteria in environmental samples was demonstrated by developing a working method for detection of muramic acid (universally found in bacteria) in airborne dust. A prototype approach for the rapid (< 10 minutes) detection/identification of microorganisms (B. anthracis) based upon the combination of polymerase chain reaction (PCR) and electrospray mass spectrometry is also described. Extrapolation of this approach to environmental monitoring would represent a major improvement over existing technologies for biodetection.

Descriptors :   *BACTERIA, *TAXONOMY, *NUCLEIC ACIDS, METHODOLOGY, ENVIRONMENTS, DETECTION, MONITORING, STATE OF THE ART, CHEMICALS, MOLECULES, POLYMERIZATION, AIRBORNE, PROTOTYPES, SEQUENCES, IDENTIFICATION, DUST, FEASIBILITY STUDIES, SEARCHING, SAMPLING, MARKERS, BIOLOGICAL DETECTION, PATHOGENIC MICROORGANISMS, CHAIN REACTIONS, MICROORGANISMS, MASS SPECTROMETRY, INDIRECT COSTS.

Subject Categories : Biochemistry
      Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE