Accession Number : ADA315216

Title :   Chronic Toxicity Studies on 1,3,5-Trinitrobenzene in Fischer 344 Rats.

Descriptive Note : Final rept. 1 May 93-30 Apr 95,

Corporate Author : ENVIRONMENTAL PROTECTION AGENCY CINCINNATI OH RISK REDUCTION ENGINEERING LAB

Personal Author(s) : Reddy, Tirumuru V. ; Daniel, F. B. ; Olson, G. R. ; Wiechman, B. ; Torsella, J.

PDF Url : ADA315216

Report Date : FEB 1996

Pagination or Media Count : 806

Abstract : Chronic toxic effects of I ,3,5-trinitrobenzene (TNB) in male and female Fischer rats were evaluated by feeding certified powdered laboratory chow diet supplemented with varied concentrations of TNB (0, 5, 60 and 300 mg/kg diet). The study was designed to accommodate three interim sacrifices (10 rats/group/sex) at 90, 180 and 365 days. The final sacrifice was performed after two years. All data related to these interim sacrifices are presented independently in appendices J to L. The calculated average TNB consumption for females was 0.23, 2.68 and 13.31 mg/kg/day and was 0.22, 2.64 and 13.44 mg/kg/day for males. Terminal body weights were significantly decreased in both sexes in the 300 mg/kg group. Relative spleen weights were decreased in both sexes in the 300 mg/kg group while brain weights were increased in females in this same group. Methemoglobin was increased in both sexes in the 300 mg/kg group while other hematological effects noted at the interim sacrifice times were not evident at two years. Histopathological examinations suggested treatment related changes in both sexes involving the kidneys (cytoplasmic/hyaline droplets) in the 60 and 300 mg/kg groups and the spleen (erythroid cell hyperplasia and pigment deposition) in the 300 mg/kg group. The cytoplasmic/hyaline droplets were characterized by immunohistochemistry as alpha-2u-globulin. These renal droplets were also noted at the interim sacrifice times. A no observed adverse effect level (NOAEL) was established in this study at 2.68 mg/kg b.w./day for F-344 rats administered TNB for two years.

Descriptors :   *BENZENE, *TOXICITY, *METHEMOGLOBIN, BRAIN, RATS, POWDERS, ABSTRACTS, CHEMISTRY, DEPOSITION, DROPS, WEIGHT, ADVERSE CONDITIONS, LABORATORIES, MALES, IMMUNOLOGY, PIGMENTS, DIET, BODY WEIGHT, FEMALES, SEX, SPLEEN, HISTOLOGY, KIDNEYS, HEMATOLOGY.

Subject Categories : Toxicology
      Inorganic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE