Accession Number : ADA315300

Title :   Physiology, Biochemistry, and Genetics of a Pure Culture of an Obligatory Anaerobic Bacterium That Utilizes 2,4,-6-Trinitrotoluene (TNT) and Biodegradation of RDX by Pure Cultures of Obligatory Anaerobic Bacteria of the Genus Clostridium.

Descriptive Note : Final rept. 1 Sep 93-31 Aug 96,

Corporate Author : IDAHO UNIV MOSCOW CENTER FOR HAZARDOUS WASTE REMEDIATION RESEARCH

Personal Author(s) : Crawford, Ronald L. ; Crawford, Don L.

PDF Url : ADA315300

Report Date : 01 SEP 1996

Pagination or Media Count : 16

Abstract : In work supported by the US AFOSR (grant F49620-94-1-0306) we are conducting detailed biochemical and genetic studies of three strains of Clostridium bifernientans, obligatory anaerobic bacteria that appear to completely degrade a variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene (TNT). We are determining the optimal physiological conditions for the degradative activities of C. bifermentans strains; and identifying and characterizing enzymes and genes involved in the biotransformation of nitroaromatic compounds by C. bifermentans. In our AASERT supplemental grant(AFOSR-93-1-O464) we expanded these goals to the explosive RDX (1,3,5-triaza-1,3,5-trinitrocyclohexane. The AASERT grant funded two graduate students, who characterized the ability of C. bifermentans to degrade RDX (Regan, K. N., and R.L. Crawford, 1994. Biotechnol. Kett. 16: 1081- 1086), and prepared both genomic and plasmid DNA libraries from C. bifermentans. This genetic work will accelerate our progress toward our goal of characterizing the genetics of TNT/RDx degradation by our clostridia (K. Diedrich, M.S. thesis, University of Idaho; in preparation).

Descriptors :   *CLOSTRIDIUM, *BIODEGRADATION, *RDX, *TNT, *NITROGEN COMPOUNDS, OPTIMIZATION, DEGRADATION, STUDENTS, BACTERIA, BIOCHEMISTRY, ENZYMES, THESES, EXPLOSIVES, DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, COSTS, CODING, PURITY, SOILS, PHYSIOLOGICAL EFFECTS, GENES, PHYSIOLOGY, UNIVERSITIES, AROMATIC COMPOUNDS, LIBRARIES, CULTURE, GENETICS, CULTURES(BIOLOGY), ANAEROBIC BACTERIA, ANAEROBIC PROCESSES, IDAHO.

Subject Categories : Microbiology
      Ammunition and Explosives

Distribution Statement : APPROVED FOR PUBLIC RELEASE