Accession Number : ADA315375
Title : AASERT-92 Research Training for Understanding the Fate of Environmental Pollutants.
Descriptive Note : Final rept. 1 Jun 93-31 May 96,
Corporate Author : CORNELL UNIV ITHACA NY
Personal Author(s) : Madsen, Eugene L.
PDF Url : ADA315375
Report Date : 31 MAY 1996
Pagination or Media Count : 5
Abstract : This award supported the training of two graduate students at Cornell University in their efforts to apply geochemical, physiological, and genetic approaches to understanding the fate of naphthalene in groundwater and sediments at a coal-tar contaminated field site. Three major scientific goals were accomplished. First, we extended methods and criteria for proving that environmental contaminants are actively being biodegraded in real time by using gas chromatography/mass spectrometry to detect a transient intermediate metabolite (1,2-dihydroxy-1,2-dihydronaphthalene) in site waters. Second, we applied molecular procedures to extend understanding of genetic processes that affect environmental contaminants by extracting DNA from site sediments, using PCR to amplify the naphthalene catabolic gene, and contrasting restriction digests of the site-and pure culture-derived genes. Third, using a combination of field and laboratory procedures, we tested several hypotheses for explaining the persistence of naphthalene in the field site. We concluded that in situ oxygen limitation and reduced bioavailability were the causes.
Descriptors : *AIR POLLUTION, *ENVIRONMENTAL IMPACT, *GROUND WATER, *WATER POLLUTION, *CONTAMINANTS, TRANSIENTS, ENVIRONMENTS, STUDENTS, REAL TIME, WATER, MASS, SITES, DEOXYRIBONUCLEIC ACIDS, OXYGEN, LIMITATIONS, LABORATORY PROCEDURES, EXTRACTION, GENES, POLLUTANTS, HYPOTHESES, SEDIMENTS, GENETICS, GAS CHROMATOGRAPHY, METABOLITES, CATABOLISM, MASS SPECTROMETRY, NAPHTHALENES.
Subject Categories : Solid Wastes Pollution and Control
Water Pollution and Control
Distribution Statement : APPROVED FOR PUBLIC RELEASE