Accession Number : ADA315786

Title :   Effect of Hormone Replacement Therapies and Dietary Phytoestrogens on the Mammary Gland of Macaques.

Descriptive Note : Annual rept. 1 Jul 95-30 Jun 96,

Corporate Author : BOWMAN GRAY SCHOOL OF MEDICINE WINSTON-SALEM NC

Personal Author(s) : Cline, J. M.

PDF Url : ADA315786

Report Date : JUL 1996

Pagination or Media Count : 71

Abstract : The purpose of this study was to use histomorphometric and immunohistochemical techniques to study the incidence and characteristics of mammary gland hyperplasia, dysplasia and other possible indicators of breast cancer risk, in cynomolgus macaques given long-term hormonal treatments. Treatments evaluated to date include conjugated estrogens (CEE), medroxyprogesterone acetate (MPA), the combination of CEE and MPA, tamoxifen, estradiol (E2), 17 alpha-dihydroequilenin (DHEN), soybean phytoestrogens (SBE), and SBE + E2. Pathologic evaluation, histomorphometric evaluations, and staining for estrogen receptor, progesterone receptor, and the proliferation marker Ki-67 MIB were done. Results are as follows: The addition of MPA to CEE therapy increases, rather than decreases, mammary gland proliferation. Tamoxifen treatment does not induce mammary gland proliferation beyond that seen in controls; this is in contrast to a marked uterotrophic effect. DHEN does not induce mammary gland or endometrial proliferation, relative to controls and in contrast to E2. Soybean estrogens do not induce mammary or endometrial proliferation when given alone, and exert an antagonistic effect on E2-induced proliferation in both sites. New work includes dietary modulation of-hormonal effects on mammary gland, identification of p53 expression in CEE-treated macaque mammary gland, and development of whole-mount techniques.

Descriptors :   *HORMONES, *MAMMARY GLANDS, *ESTROGENS, *MACAQUE MONKEYS, *BREAST CANCER, TEST AND EVALUATION, NUCLEAR PROLIFERATION, CONTROL, RISK, LONG RANGE(TIME), REPLACEMENT, THERAPY, PATHOLOGY, IMMUNOLOGY, DIET, INDICATORS, SENSE ORGANS, HISTOLOGY, SOYBEANS, PROGESTERONE, DYSPLASIA.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE