Accession Number : ADA315807

Title :   Ligands for Stretch Activated Ion Channels.

Descriptive Note : Final rept.,

Corporate Author : STATE UNIV OF NEW YORK AT BUFFALO AMHERST

Personal Author(s) : Sachs, Frederick

PDF Url : ADA315807

Report Date : MAY 1996

Pagination or Media Count : 6

Abstract : During tenure of the grant we have made tremendous strides in finding active ligands for the mechanosensitive channels. We developed a simple screen for the toxins based upon hypotonic swelling of GH3 clonal neurons which produced increases in internal Ca2+ levels that could be measured using Fura-2. Addition of active venoms would lead to a decrease in Ca2+ levels following swelling. Screening a variety of spider and scorpion venoms, we found that none of the scorpions tested (= 12) but one of the 8 spiders tested was able to block volume activated Ca2+ uptake. The raw venom also blocked stretch activated ion channels in Xenopus oocytes, chick heart cells and GH3 cells, and whole cell mechanical currents in chick heart cells.

Descriptors :   *LIGANDS, *CHICKENS, *TOXINS AND ANTITOXINS, IONS, MECHANICAL PROPERTIES, ACTIVATION, CELLS, NERVE CELLS, CHANNELS, VENOMS, CURRENTS, HEART.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE