Accession Number : ADA315955

Title :   Role of a Novel Antioncogene that Prevents Metastatic Spread of Disease.

Descriptive Note : Annual rept. 1 Jul 95-30 Jun 96,

Corporate Author : BOWMAN GRAY SCHOOL OF MEDICINE WINSTON-SALEM NC

Personal Author(s) : Kute, Timothy E.

PDF Url : ADA315955

Report Date : JUL 1996

Pagination or Media Count : 46

Abstract : The purpose of this grant is to determine a mechanism of action for a novel antioncogene (i.e. nm23) which prevents the metastatic spread of disease. Our data would indicate that there is no relationship of nm23 expression with proteolytic factors such as cathepsin D, urokinase plasminogen activator, its receptor and its inhibitor. High levels of these factors have been shown to predict metastatic disease in breast cancer patients. Both western blot and ELISA analyses and also by immunohistochemistry were used to prove this lack of correlation. We did show that nm23 expression was related to metastatic potential using cell lines and xenographs. We also performed a clinical trial to determine if nm23 could predict prognosis in node negative breast cancer patients. Our results indicate that nm23 is not a independent prognostic indicator. Our results do indicate that when MDA-MB-23 1 cells are transfected with nm23 gene, they have a lower motility and become less metastatic in the nude mouse model system.

Descriptors :   *CLINICAL MEDICINE, *INFECTIOUS DISEASES, *PREVENTIVE MEDICINE, *METASTASIS, *BREAST CANCER, ACTIVATION, PREDICTIONS, MODELS, ENZYMES, DISEASES, PLASMIDS, CHEMISTRY, PATIENTS, MICE, IMMUNOLOGY, IMMUNOASSAY, HISTOLOGY, ANATOMICAL MODELS, ENZYME PRECURSORS, PHOSPHORUS TRANSFERASES.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE