Accession Number : ADA316448
Title : New Multilabel Fluorescent Groups for Increased Sensitivity of DNA Detection.
Descriptive Note : Final progress rept. 1993-96,
Corporate Author : ROCHESTER UNIV NY DEPT OF CHEMISTRY
Personal Author(s) : Kool, Eric T.
PDF Url : ADA316448
Report Date : 01 OCT 1996
Pagination or Media Count : 8
Abstract : The long-term goals of this project are to use oligonucleotides tagged with multiple fluorescent labels as hybridization probes of specific nucleic acid sequences. We have developed novel modes of binding between designed DNA probes and target DNA or RNA sequences, and we are investigating the combination of these new binding modes with dual (or more) fluorescent labels to raise signal intensity and signal-to-noise ratios. Such probes may be useful in detection and identification of pathogen nucleic acids as well as disease-related nucleic acid sequences in humans.
Descriptors : *DETECTION, *FLUORESCENCE, *DEOXYRIBONUCLEIC ACIDS, *HYBRIDIZATION, PROBES, HUMANS, SIGNAL TO NOISE RATIO, INTENSITY, TARGETS, SENSITIVITY, SEQUENCES, SIGNALS, RIBONUCLEIC ACIDS, LABELS, NUCLEIC ACIDS.
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE