Accession Number : ADA317023

Title :   Identification of Brucella Melitensis Antigens by T Lymphocytes and Oral Immunization Potential of the Antigens.

Descriptive Note : Final rept. 1 Jun 94-31 May 96,

Corporate Author : WISCONSIN UNIV-MADISON

Personal Author(s) : Splitter, Gary

PDF Url : ADA317023

Report Date : JUL 1996

Pagination or Media Count : 84

Abstract : Acquired immunity against intracellular bacteria is T cell dependent. T cells play a major role in protection against intracellular bacteria, but bacterial antigens recognized by T cells have been studied less extensively than bacterial antigens recognized by B cells. Using T lymphocytes from Brucella abortus immunized animals, we have screened a bacterial genomic library for genes encoding antigens recognized by T cells. Lymphocytes that proliferated to B. abortus proteins were characterized for phenotype and cytokine activity. Lymphocytes recognizing bacterial antigens possessed type 1 cytokine profiles, i.e., IFN-gamma and IL-2 but not IL-4 production. Lymphocytes necesssary for In vivo protection were examined using gene knockout mice. MHC class I and class II gene knockout animals infected with B. abortus demonstrated that protection to B. abortus is especially dependent on CD8+ T cells. Knowing the cells required for protection, vaccines can be designed to elicit the protective subset of lymphocytes. Several recombinant B. abortus proteins were tested using different immunization strategies.

Descriptors :   *BACTERIA, *T LYMPHOCYTES, *ANTIGENS, *BRUCELLA MELITENSIS, *BRUCELLA ABORTUS, STRATEGY, PROTEINS, CODING, PROTECTION, GENES, ANIMALS, MICE, IMMUNITY, IN VIVO ANALYSIS, CELLS(BIOLOGY), IMMUNIZATION, LYMPHOCYTES, ORAL INTAKE, VACCINES.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE