Accession Number : ADA317280

Title :   PCR and Mammalian Cell Selection Assays for Short-Term Genotoxicity Testing Background.

Descriptive Note : Final rept. 24 Feb 92-23 Aug 96,

Corporate Author : TULANE UNIV MEDICAL CENTER NEW ORLEANS LA

Personal Author(s) : Ehrlich, Melanie

PDF Url : ADA317280

Report Date : SEP 1996

Pagination or Media Count : 30

Abstract : In order to develop an extremely sensitive test for chemicals that induce chromosomal rearrangements, a polymerase chain reaction (PCR) assay has been optimized for the single- molecule detection of a translocation-containing human DNA sequence. We have used this assay for rearrangements between the bcl-2 proto-oncogene and the JH immunoglobulin gene region to test for this lymphoma-associated translocation in genotoxin-treated cultured cells and in peripheral blood mononuclear cells. Our most important findings from this study are that approximately half of healthy adults contain this translocation in their blood at levels detectable with our ultrasensitive assay; that there is an age-related increase in the frequency of this translocation; and that some individuals have much higher levels of this translocation than their age-mates. This may reflect environmentally hazardous exposures or genetic defects and an increased risk of developing cancer.

Descriptors :   *MAMMALS, *TOXICITY, *DEOXYRIBONUCLEIC ACIDS, *CELLS(BIOLOGY), *CHAIN REACTIONS, TEST AND EVALUATION, DETECTION, RISK, CHEMICALS, MOLECULES, POLYMERIZATION, SENSITIVITY, DEFECTS(MATERIALS), SELECTION, ASSAYING, GENETICS, ADULTS, NUCLEI, CANCER, BLOOD VOLUME, BLOOD CELLS, HIGH SENSITIVITY, TRANSLOCATION.

Subject Categories : Biochemistry
      Medicine and Medical Research
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE