Accession Number : ADA317374

Title :   Stromal Components of Breast Cancer Progression.

Descriptive Note : Annual rept. 15 Jun 95-14 Jun 96,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : McLeskey, Sandra W.

PDF Url : ADA317374

Report Date : JUL 1996

Pagination or Media Count : 19

Abstract : Metastatic estrogen receptor positive breast carcinoma may be treatable with tamoxifen, an antiestrogen, but the tumor may subsequently become refractory to treatment, growing in the absence of estrogenic stimulation. We have developed a model of breast cancer progression by transfecting MCF-7 breast carcmoma cells, which are estrogen-dependent for growth in ovariectomized nude mice, with cDNAs for FGF-I or FGF-4. The transfected cell lines are able to form tumors in ovariectomized nude mice. Since FGFs are angiogenic factors, this project investigates the importance of angiogenesis in the phenotypic transition of the transfected cells by looking for differences in the angiogenesis in tumors produced by the parental MCF-7 or the FGF-transfected cells. Our model is validated by a positive correlation of tumor microvessel density and tumor size. We have defined temporal and spatial events in the process of tumor-induced angiogenesis by identifing sprouting or proliferating endothelial cells. We have identified patterns of angiogenesis which are associated with regressing parental cell tumors and growing FGF-transfected cell tumors. We have begun to isolate endothelial cell populations from parental or FGF-transfected cell tumors to study FGF receptor gene expression.

Descriptors :   *BREAST CANCER, STIMULATION(PHYSIOLOGY), MODELS, NEOPLASMS, GENES, MICE, CELLS(BIOLOGY), RECEPTOR SITES(PHYSIOLOGY), CANCER, ENDOTHELIUM, ESTROGENS, METASTASIS.

Subject Categories : Genetic Engineering and Molecular Biology
      Biology
      Medicine and Medical Research
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE