Accession Number : ADA318009
Title : Augmentation Award for Monoclonal Antibody Detection of Chlorinated Benzenes on Contaminated Sediments.
Descriptive Note : Final rept. 1 Sep 93-31 Aug 96,
Corporate Author : MISSOURI UNIV-COLUMBIA DEPT OF CIVIL ENGINEERING
Personal Author(s) : Mossman O'Bannon, Deborah J.
PDF Url : ADA318009
Report Date : 31 OCT 1996
Pagination or Media Count : 4
Abstract : The nonextractive immunoassay techniques developed with enzyme immunoassay procedures were extended to include fluorescent immunoassay of sorbed contaminants. The variety of substrates to which the contaminant was sorbed was increased to include: St. Peters sandstone, montmorillonite, and black shale. Auto-fluorescence of the substrates was addressed, and 2,4-dinitro-benzene was found not to migrate into the montmorillonite crystal layers. Additional sample preparation was needed for the clay samples: significantly more blocking solution was needed to reduce background nonspecific binding of the immunochemicals. Two contaminants were used throughout the grant period -- 2-4-dinitrobenzene, and pyrene.
Descriptors : *BENZENE, *FLUORESCENCE, *ENZYMES, *CONTAMINATION, *MONOCLONAL ANTIBODIES, METHODOLOGY, DETECTION, LAYERS, AUGMENTATION, SUBSTRATES, CRYSTALS, SOLUTIONS(GENERAL), SAMPLING, CLAY, SANDSTONE, BACKGROUND, CONTAMINANTS, BLOCKING, IMMUNOASSAY, CHLORINATION, SEDIMENTS, BLACK(COLOR), SHALE, MONTMORILLONITE.
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE