Accession Number : ADA318234

Title :   Structural and Functional Studies of Experimental HIV Synthetic Peptide Immunogens.

Descriptive Note : Annual rept. 30 Sep 95-29 Sep 96,

Corporate Author : DUKE UNIV MEDICAL CENTER DURHAM NC

Personal Author(s) : Haynes, Barton F.

PDF Url : ADA318234

Report Date : OCT 1996

Pagination or Media Count : 126

Abstract : This grant addresses 2 major problems in HIV synthetic peptide vaccine development: (1) the ability of synthetic peptides to mimic conformational determinants of HIV envelope proteins, and (2) the design of optimally immunogenic multivalent peptide immunogens capable of being recognized by MllC Class I and II types of outbred populations. In Technical Aim (T.A.) #1, a peptide mimitope of the 48d human mab has been found that binds to fusin+ T cells, and in binding to human cells, ceases exposure of the 48d binding site. Immunogenic gpi2O C4-V3 peptides have been mutated based on structural predictions that improve the immunogenicity of C4-V3 peptides. New potentially neutralizing determinant peptides from HIV gpi2O and gp4i have been made and are being tested in a novel water based adjuvant strategy utilizing intranasal immunization for optimal systemic antibody response generation. In T.A.#2, the 4 C4-V3 peptides from HIV MN, RF, EV9i and CANOA as well as C4 mutant peptides have been studied using NMR, their solution conformers determined, and structure-function relationships made with studies in T.A.#1. In T.A.#3, an assay has been developed to predict HIV peptide binding to specific HLA molecules.

Descriptors :   *PEPTIDES, *SYNTHESIS(CHEMISTRY), *T LYMPHOCYTES, *HUMAN IMMUNODEFICIENCY VIRUSES, *IMMUNIZATION, *VACCINES, HUMANS, MOLECULES, STRUCTURAL PROPERTIES, WATER, PROTEINS, CELLS(BIOLOGY), ENVELOPE(SPACE), IMMUNOGENS, NOSE(ANATOMY).

Subject Categories : Biochemistry
      Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE