Accession Number : ADA318579

Title :   A Novel Model System to Examine Agents Used in Breast Cancer Therapy.

Descriptive Note : Annual rept. 1 Jul 95-30 Jun 96,

Corporate Author : MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE

Personal Author(s) : Coll, Jennifer ; Malkas, Linda H.

PDF Url : ADA318579

Report Date : JUL 1996

Pagination or Media Count : 113

Abstract : We have recently described a multiprotein complex for DNA replication (DNA synthesome) isolated from MDA MB 468 human breast cancer cells, human breast tumor tissue and human breast tumor cell xenografts In the presence of the viral large T-antigen and simian virus 40 (SV40) origin sequences, the DNA synthesome executes all of the steps required for the in vitro replication of the SV40 genome. Furthermore, the DNA synthesome isolated from human breast cancer cells possesses a lower fidelity for DNA synthesis in vitro than the synthesome purified from a non-malignant breast cell line. Our studies indicate that the following DNA replication proteins constitute the DNA synthesome: DNA polymerase alpha primase, DNA polymerase delta, DNA polymerase epsilon, proliferating cell nuclear antigen (PCNA), RF-C, RP-A and DNA topoisomerases I, II.

Descriptors :   *MODELS, *THERAPY, *BREAST CANCER, TISSUES(BIOLOGY), HUMANS, ENZYMES, PROTEINS, DEOXYRIBONUCLEIC ACIDS, IN VITRO ANALYSIS, HUMAN BODY, REPLICAS, VIRUSES, CELLS(BIOLOGY), ANTIGENS, BIOSYNTHESIS, MAMMARY GLANDS, NUCLEOTIDES.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE