Accession Number : ADA318714

Title :   The Effect of Room Temperature Storage on the in Vitro Storage Characteristics of CPDA-1 Packed Red Blood Cells.

Descriptive Note : Master's thesis,

Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH

Personal Author(s) : Ruddell, Jean P.

PDF Url : ADA318714

Report Date : AUG 1996

Pagination or Media Count : 91

Abstract : Packed red blood cells (PRBC) which reach temperatures exceeding 10 deg C are generally discarded. Little data exist on the degree of accelerated metabolism and increased hemolysis of PRBC allowed to warm for more than a few minutes. Twenty four CPDA- 1 PRBC units were pooled in eight groups of three. Each pool was divided into two test units and a control unit. Test units were warmed to 250C for 24 hours either at day 6 or day 20; controls were maintained at 1 -60C. In-vitro storage characteristics were evaluated weekly and prior to warming. Sterility was evaluated at day 35. Data were analyzed by ANOVA. Control units had higher ATP and glucose, less hemolysis, and equivalent morphology to the warmed test units at day 35. Warmed units had adequate ATP and glucose, equivalent hemolysis, and better morphology at day 28 than adequate ATP and glucose, equivalent hemolysis, and better morphology at day 28 than day 35 control units. With the exception of ATP, test units at day 28 were equivalent to or better than controls at day 35. In the day 28 warmed units, ATP exceeded 1.5 %mole/g hemoglobin (Hgb). No bacterial growth was detected despite repeated sampling. It appeared that a day of 250C storage of CPDA-l PRBC accelerated aging equivalent to a week of conventional storage at l-60C. It did not appear to matter whether the PRBC were warmed at day 6 or day 20. Medical directors may find this information useful in logistically difficult circumstances.

Descriptors :   *IN VITRO ANALYSIS, *ROOM TEMPERATURE, *BLOOD CELLS, *BLOOD STORAGE, *HEMOLYSIS, CONTROL SYSTEMS, AGING(MATERIALS), BACTERIA, MORPHOLOGY, TEST EQUIPMENT, METABOLISM, HEATING, SAMPLING, STORAGE, ERYTHROCYTES, ACCELERATED TESTING, GLUCOSE, STERILIZATION, GROWTH(PHYSIOLOGY).

Subject Categories : Medicine and Medical Research
      Thermodynamics

Distribution Statement : APPROVED FOR PUBLIC RELEASE