Accession Number : ADA318731

Title :   Methodology To Determine Homology and Clustering as Applied To Intronic Regions of Regulatory Cancer Genes and Non-Regulatory Genes.

Descriptive Note : Doctoral thesis,

Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH

Personal Author(s) : Hall, Deborah L.

PDF Url : ADA318731

Report Date : 31 MAY 1996

Pagination or Media Count : 171

Abstract : This thesis considers the problem of statistically evaluating the closeness of multiple genes based on their DNA intronic regions. The purpose of intronic regions in a gene is unknown. Recent research suggests that, in cancer related genes, intronic regions may play a role in regulating disease susceptibility. To investigate whether the intronic features of cancer related genes differ from non-regulatory genes, a collection of oncogenes, tumor suppressor genes, and non- regulatory genes involved in enzyme metabolism are analyzed. A statistical methodology is employed to determine whether features of these genes' intronic regions will result in clustering by regulatory group. This is the first comparison of intronic attributes for 33 homo sapien genes. Affributes analyzed include mean of intronic log lengths, standard deviations of intronic log lengths and number of intronic regions in a gene. The analysis also includes the available ordered DNA data from these intronic regions. The process begins with creation of first-order Markov transition counts for all intronic regions in each gene. A hypothesis testing approach employs these counts to determine whether the order of the nucleotides in each intronic region is random or whether this order shows statistical evidence of a first-order Markov chain.

Descriptors :   *NEOPLASMS, *GENES, *GENETICS, *CANCER, TEST AND EVALUATION, METHODOLOGY, ENZYMES, COMPARISON, STATISTICAL DATA, STATISTICS, DISEASES, THESES, METABOLISM, DEOXYRIBONUCLEIC ACIDS, HYPOTHESES, COLLECTION, STANDARD DEVIATION, MARKOV PROCESSES, SUPPRESSORS, NUCLEOTIDES.

Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE