Accession Number : ADA319341

Title :   Role of the Int-3 Oncogene in Mammary Gland Development and Tumorigenesis.

Descriptive Note : Annual rept. 15 Aug 95-14 Aug 96,

Corporate Author : COLUMBIA UNIV NEW YORK

Personal Author(s) : Kitajewski, Jan ; Uyttendaele, Hendrik

PDF Url : ADA319341

Report Date : SEP 1996

Pagination or Media Count : 35

Abstract : The overall goal of the work proposed here is to understand in molecular detail the function of the int-3 protein in mammary epithelial cells and during mouse mammary gland development. The approach I propose will have two major objectives. First, the biochemical and biological properties of the int-3 protein will be investigated. I have cloned the part of the int-3 gene that encodes for the putative intracellular domain of the int-3 protein, which is thought to be constitutively active. Initially, this truncated protein will be used as a probe to study the signaling pathway and the mechanisms of action of the int-3 protein. These studies will be further expanded once the full-length int-3 is cloned. Second, I will clone, sequence and characterize the full length int-3 cDNA and study int-3 function and expression pattern in mammary epithelial cells as well as during mammary gland development.

Descriptors :   *MAMMARY GLANDS, *BREAST CANCER, PROBES, EPITHELIUM, BIOCHEMISTRY, PROTEINS, DEOXYRIBONUCLEIC ACIDS, CODING, PATTERNS, GENES, CLONES, MICE, CELLS(BIOLOGY), TRUNCATION, BIOMOLECULES.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE