Accession Number : ADA319492

Title :   Genotoxicity Assays Of Ammonium Dinitramode I. Salmonella/Microsome Mutagenesis II. Mouse Lymphoma Cell Mutagenesis III. In Vivo Mouse Bone Marrow Micronuclei Test.

Descriptive Note : Final rept. Mar-Jul 94,

Corporate Author : MANTECH ENVIRONMENTAL TECHNOLOGY INC RESEARCH TRIANGLE PARK NC

Personal Author(s) : Zhu, S. ; Korytynski, E. ; Sharma, S.

PDF Url : ADA319492

Report Date : NOV 1994

Pagination or Media Count : 79

Abstract : Ammonium dinitramide (ADN) was examined for its genetic toxicology effects using a battery of short-term mutagenicity screening assays, which included Salmonella/microsome mutagenesis (Ames test), mouse lymphoma cells mutagenesis (L5178Y-TK test), and in vivo mouse bone marrow micronuclei assay. Results of Ames test indicated that ADN was a base-pair substitution mutagen, causing about 2-fold (without S9) or 3-fold (with S9) increases of revertants in TA 100, while there was 0 increase of mutants in TA1535, TAl537, and TA98. ADN also induced mutation at the TK locus bf mouse lymphoma cells, causing 40-95% (without S9) or 130-220% (with S9) increases of mutants. The in vivo micronuclei examination revealed a dose-dependent increase of micronucleated cells in the bone marrow of both male and female mice treated with ADN in a dose range of 62.5-750 mg/kg (single dose for 3 consecutive days), with a maximal induction of 3-fold increase at the highest dose. Toxicity (determined as a decrease in PCE/NCE ratio) was observed in the same dose range. These results demonstrate that ADN is mutagenic to both bacteria and mammalian cells and causes chromosomal damage in mouse bone marrow cells in vivo.

Descriptors :   *TOXICITY, *GENETICS, *SALMONELLA, *BONE MARROW, *TOXICOLOGY, *LYMPHOMAS, RATIOS, MAMMALS, BACTERIA, MUTATIONS, CHROMOSOMES, MICE, IN VIVO ANALYSIS, CELLS(BIOLOGY), LOCUS.

Subject Categories : Medicine and Medical Research
      Toxicology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE