Accession Number : ADA319735

Title :   Fidelity of DNA Replication in Normal and Malignant Human Breast Cells.

Descriptive Note : Annual rept. 1 Aug 95-31 Jul 96,

Corporate Author : MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE

Personal Author(s) : Weeks, Jennifer

PDF Url : ADA319735

Report Date : AUG 1996

Pagination or Media Count : 32

Abstract : In order to better understand the extent to which the intact DNA replication machinery contributes to the overall mutation frequencies observed in normal and malignant breast cells, I have designed experiments to examine the degree of fidelity exhibited during the DNA replication process in both normal and cancerous breast cells. To accomplish this goal I have isolated a multiprotein DNA replication complex (which we have designated the DNA synthesome) from both normal breast tissue cells and malignant breast cancer cells and have begun to determine the ability of the DNA synthesome from both cell types to faithfully copy a target gene used in our in vitro replication assay system.

Descriptors :   *BREAST CANCER, TISSUES(BIOLOGY), HUMANS, PROTEINS, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, IN VITRO ANALYSIS, GENES, CELLS(BIOLOGY), MAMMARY GLANDS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE