Accession Number : ADA319783

Title :   Cloning of Human Monoclonal Antibodies Associated with Medullary Ductal Carcinoma.

Descriptive Note : Final rept. 15 Aug 95-14 Aug 96,

Corporate Author : NEW ENGLAND DEACONESS HOSPITAL BOSTON MA

Personal Author(s) : Telleman, Peter

PDF Url : ADA319783

Report Date : SEP 1996

Pagination or Media Count : 15

Abstract : Combinatorial phage technology is used to identify and characterize newly expressed proteins which elicit plasma cell reactions in certain breast carcinomas. It is our principal hypothesis that this reaction reflects a local immune response against tumor that is etiologic in the historical impression of a more favourable natural course of these tumors after surgery-only therapy. DNA sequencing of IgG H and L chains from random unselected clones indicates a dramatic focus in libraries derived from two patients with medullary ductal carcinoma, supporting our hypothesis of a local immune response in these tumors. Reactive phage clones are selected from phage display libraries using optimal cell-based panning conditions with medullary ductal carcinoma cells. It was demonstrated that Her2/neu and p53 are not the eliciting antigens.We will be employing immunoprecipitation and immunoblotting in order to isolate the antigen of interest.

Descriptors :   *ANTIBODIES, *CARCINOGENS, *MAMMARY GLANDS, *BREAST CANCER, PLASMAS(PHYSICS), NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, CLONES, HYPOTHESES, PATIENTS, RESPONSE(BIOLOGY), GENETIC ENGINEERING, IMMUNITY, CELLS(BIOLOGY), IMMUNOASSAY, REPRODUCTION(PHYSIOLOGY), BACTERIOPHAGES.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE