Accession Number : ADA319847

Title :   Suppression of Lymphocyte Signal Transduction by Oxygen Intermediates.

Descriptive Note : Annual rept. 30 Sep 95-29 Sep 96,

Corporate Author : NEW YORK UNIV MEDICAL CENTER TUXEDO PARK LAB OF ENVIRONMENTAL STUDIES

Personal Author(s) : Flescher, Eliezer

PDF Url : ADA319847

Report Date : OCT 1996

Pagination or Media Count : 27

Abstract : Oxidative stress alters the expression of transcription factors in human lymphocytes. Given the critical role these factors play in gene regulation, their abnormal regulation should lead to disturbances in gene expression and these in turn will result in cellular dysfunction. The transcription factor abnormalities may be used to develop an assay for the detection of environmental toxic oxidants. Technical objectives: To study three models of inducing oxidative stress in lymphocytes: (a) PAO activity generating extracellularly low levels of H2O2 for two days (mimicking exposures to environmental chemical toxicants); (b) electron irradiation generating both extra and intracellularly mainly OH; and (c) high levels of reagent H2O2 generating short but acute stress. The following questions were asked during the first year of work: Do human lymphocytes subjected to oxidative stress: (a) exhibit abnormal cellular function-expression of transcription factors that regulate the interleukin 2 gene (essential for T lymphocyte function)?; and (b) sustain damage as assessed by an independent and established method: measurement of lipid peroxidation?

Descriptors :   *LYMPHOCYTES, HUMANS, TOXICITY, LIPIDS, STRESS(PHYSIOLOGY), OXYGEN, OXIDATION, OXIDIZERS, GENES, ELECTRON IRRADIATION, CELLS(BIOLOGY), HYDROGEN PEROXIDE.

Subject Categories : Biochemistry
      Medicine and Medical Research
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE