Accession Number : ADA319887

Title :   Composition of Coal Dusts and Their Cytotoxicity on Alveolar Macrophages.

Descriptive Note : Final rept.,

Corporate Author : ARMY ARMAMENT RESEARCH DEVELOPMENT AND ENGINEERING CENTER WATERVLIET NY BENET LABS

Personal Author(s) : Lee, C. Y. ; Lee, S. L. ; Sheehan, C. E. ; Wang, Y.

PDF Url : ADA319887

Report Date : SEP 1996

Pagination or Media Count : 18

Abstract : Coal mine dust is produced from complex materials consisting of organic sedimentary strata, inorganic minerals, and trace elements. The dust varies in its chemical compositions and is capable of causing lung injury and damage when inhaled. The purpose of this study was to perform scanning electron microscopy combined with energy-dispersive spectrometry, wavelength-dispersive spectrometry, and X-ray diffraction analyses of three coal dusts, and examine their effects on rat lung alveolar macrophages (AMs) in cell culture. The coal dusts were obtained from coal surfaces of anthracite, meager, and fat coal mines. The AMs were harvested in bronchoalveolar lavage from adult male Wistar rats and were cultured in Eagle's medium at 37 deg C. Prostaglandin E2 (PGE2) and lactate dehydrogenase (LD) released by cultured AMs were measured by radioimmunoassay and enzymatic methods, respectively, 24 hours after addition of coal dust. Cytotoxicity was evident in AM culture of all three coal dusts, which caused the release of LD and PGE2. The release was dose-dependent. In summary, our study shows that all three coal dusts exhibit cytotoxicity to AMs and suggests that the pathogenesis of coal associated with pulmonary disease may be linked to the elemental compositions and mineralogic components.

Descriptors :   *CYTOTOXINS, *COAL, *ALVEOLI, SCANNING ELECTRON MICROSCOPES, RATS, ENZYMES, ENERGY, X RAY DIFFRACTION, CHEMICAL COMPOSITION, ELECTRON MICROSCOPY, DUST, LUNG, FATS, CELLS(BIOLOGY), CULTURES(BIOLOGY), PATHOGENESIS, MACROPHAGES, RESPIRATORY DISEASES, TRACE ELEMENTS, INORGANIC MATERIALS, MINERALOGY, ANTHRACITE, RADIOIMMUNOASSAY, LACTIC DEHYDROGENASE.

Subject Categories : Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE