Accession Number : ADA320024

Title :   The Role of G2/M Checkpoint Controls in Cytotoxic Treatment of Breast Cancer.

Descriptive Note : Annual rept. 15 Aug 95-14 Aug 96,

Corporate Author : SCRIPPS RESEARCH INST LA JOLLA CA

Personal Author(s) : McGowan, Clare H.

PDF Url : ADA320024

Report Date : SEP 1996

Pagination or Media Count : 16

Abstract : The objective of the work is to provide a detailed understanding of the molecular mechanism by which G2/M regulation is achieved in human cells. In particular we are focusing on how initiation of M-phase is delayed in cells that have been treated with agents that induce DNA damage or that prevent synthesis of DNA. By providing a more detailed explanation of how cytotoxic therapies brings about cell death we hope to provide clinicians with better tools for the treatment of breast cancer. Human cell-lines that over-express non-phosphorylatable mutants of CDC2 were used to establish the importance of inhibitory phosphorylation of CDC2 in checkpoint control in human cells. We are currently refining these studies and developing assays for the kinases and phosphatases that regulate the phosphorylation state of CDC2 in vivo.

Descriptors :   *CYTOTOXINS, *CELLS(BIOLOGY), *BREAST CANCER, DAMAGE, HUMANS, SYNTHESIS, MOLECULES, DEOXYRIBONUCLEIC ACIDS, THERAPY, DEATH, IN VIVO ANALYSIS, ASSAYING, PHOSPHORYLATION, PHOSPHATASES, PHOSPHORUS TRANSFERASES.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE