Accession Number : ADA320399

Title :   Oligosaccharide Markers for Prognosis of Low-Risk Breast Cancer.

Descriptive Note : Annual rept. 1 Sep 95-31 Aug 96,

Corporate Author : COLORADO UNIV HEALTH SCIENCES CENTER DENVER

Personal Author(s) : Pettijohn, David E.

PDF Url : ADA320399

Report Date : SEP 1996

Pagination or Media Count : 11

Abstract : During the period since the last progress report, the expression of several different cell surface oligosaccharides and proteases have been defined on sections from 86 different ductal breast carcinomas. Monoclonal antibodies specific for the blood group related oligosaccharides were used to define the cell surface oligosaccharides and both immunofluorescence techniques and immuno streptavadin-biotin techniques were used to evaluate the fraction of tumor cells that are positive and the relative amount of reaction product as measured using an image processing system. The preliminary analysis of the available data has shown that the extended Lea-Lex oligosaccharide is a statistically significant indicator of poor prognosis following surgical removal of small, node-negative ductal breast carcinomas. cDNA libraries constructed from cells that express Lea-Lex have been screened and selected for cDNAs that alter the expression of this oligosaccharide in cancer cells. Two candidate genes have been identified and preliminarily characterized.

Descriptors :   *CARBOHYDRATES, *BREAST CANCER, IMAGE PROCESSING, RISK, PREDICTIONS, GLYCOPROTEINS, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, NODES, DUCTS, GENES, MARKERS, MONOCLONAL ANTIBODIES, CELLS(BIOLOGY), SUGARS, MAMMARY GLANDS, PEPTIDE HYDROLASES, BLOOD GROUPS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE