Accession Number : ADA320556

Title :   Breast Cancer and Estrogen Biosynthesis in Adipose Tissue.

Descriptive Note : Annual rept. 12 Sep 95-11 Sep 96,

Corporate Author : TEXAS UNIV AT DALLAS

Personal Author(s) : Bulun, Serdar E.

PDF Url : ADA320556

Report Date : OCT 1996

Pagination or Media Count : 46

Abstract : The long-range goal of this Application was to characterize the regulation of aromatase expression in the breast bearing a tumor. It included four specific aims: to quantify adipose tissue P450arom transcript levels in a large number of mastectomy specimens at various distances from the tumor; to determine alternative promoters that are used for aromatase expression in adipose samples proximal to a tumor; to characterize novel 5'-ends of P450arom transcripts in breast cancer tissues; to characterize tumor-derived factors that induce aromatase expression in the surrounding adipose tissue. We mapped distribution of adipose P450arom transcript levels in 18 mastectomy and 11 control reduction mammoplasty specimens. The highest levels were found in the adipose tissue proximal to a tumor. In the cancer-free breast, the highest transcript levels were localized to the upper and outer regions, which are the most likely sites of tumor development. Additionally, we demonstrated that the cAMP responsive promoters, II and 1.3, are primarily used in the breast adipose bearing a tumor, whereas the cytokine/glucocorticoid-responsive promoter, 1.4, is preferentially used in the cancer-free breast. In the breast cancer tissues, promoters II and 1.3-specific transcripts comprise the majority of P450arom mRNA. The rest of the studies are detailed in the enclosed text.

Descriptors :   *BIOSYNTHESIS, *ADIPOSE TISSUE, *ESTROGENS, *BREAST CANCER, TISSUES(BIOLOGY), HUMANS, PROTEINS, NEOPLASMS, GENES, RIBONUCLEIC ACIDS, CYCLIC COMPOUNDS, MAMMARY GLANDS, ADENOSINE PHOSPHATES, NUCLEOTIDES.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE