Accession Number : ADA320651

Title :   Crystallization and Structure Determination of the Human Estrogen Receptor by X-ray Diffraction.

Descriptive Note : Annual rept. 15 Sep 95-14 Sep 96,

Corporate Author : CHILDREN'S HOSPITAL MEDICAL CENTER BOSTON MA

Personal Author(s) : Harrison, Stephen C.

PDF Url : ADA320651

Report Date : OCT 1996

Pagination or Media Count : 18

Abstract : This project is a postdoctoral training fellowship for Dr. Robert Nolte, to support X-ray crystallographic studios of cancer-related macromolecular complexes. We have prepared crystals of a complex between a six Zn-finger segment of the transcription factor TPIIIA and a 31 BP DNA site. The determination of this structure is almost complete at an intermediate resolution, with the position of the DNA and protein elements determined. A novel interaction between one of the zinc fingers and the minor groove has been observed. The higher resolution details of the structure, which will be determined in the next several months, should reveal significant now aspects of DNA recognition by Zn-finger proteins. These interactions are responsible for the regulation of many loci important for cell growth. During the final project year of this training grant, Dr. Nolte to will conclude the TFIIIA studies and focus his attention on structural studios on another protein ZAP-70, a T-cell tyrosine kinase which plays a crucial role in signal transduction.

Descriptors :   *HUMANS, *X RAY DIFFRACTION, *CRYSTALLIZATION, *ESTROGENS, *BREAST CANCER, CRYSTAL STRUCTURE, TRAINING, PROTEINS, CRYSTALS, HIGH RESOLUTION, DEOXYRIBONUCLEIC ACIDS, SIGNALS, ZINC, MACROMOLECULES, TRANSDUCERS, CELLS(BIOLOGY), TYROSINE, PHOSPHORUS TRANSFERASES.

Subject Categories : Biochemistry
      Medicine and Medical Research
      Crystallography
      Atomic and Molecular Physics and Spectroscopy
      Optics
      Nuclear Physics & Elementary Particle Physics

Distribution Statement : APPROVED FOR PUBLIC RELEASE