Accession Number : ADA321121

Title :   Enhanced Trichloroethylene Degradation Using Genetically-Engineered Microorganisms.

Descriptive Note : Final rept.,

Corporate Author : CALIFORNIA UNIV IRVINE

Personal Author(s) : Wood, Thomas K.

PDF Url : ADA321121

Report Date : 28 DEC 1996

Pagination or Media Count : 4

Abstract : Novel recombinant TCE-degrading bacteria were created using the best known enzyme for TCE degradation, soluble methane monooxygenase(sMMO) of the soil bacterium Methylosinus trichosporium OB3b. sMMO degrades a wide range of halogenated hydrocarbons (HCFCs, chloroform, dichloroethane, etc.), and it degrades TCE 100 times faster than any other microbial enzyme. The mmo genes were cloned and expressed in Pseudomonas putida Fl, Agrobacterium tumefaciens, and Rhizobium meliloti using plasmids pSMMO20 and pSMMO40 created by the Wood laboratory. In addition, a novel fixed-film bioreactor has been constructed and optimized to mineralize TCE in the gas phase.

Descriptors :   *BACTERIA, *ENZYMES, *MICROORGANISMS, *TRICHLOROETHYLENE, DEGRADATION, VAPOR PHASES, SOILS, GENES, CLONES, LABORATORIES, RANGE(EXTREMES), HALOGENATED HYDROCARBONS, WOOD, CHLOROFORM, CHLOROETHANES.

Subject Categories : Biochemistry
      Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE