Accession Number : ADA321239

Title :   Search for Genes Having TNT Degrading Capability for Bioremediation.

Descriptive Note : Final rept. 25 Oct 93-18 Sep 96,

Corporate Author : HOWARD UNIV WASHINGTON DC

Personal Author(s) : Dutta, S. K.

PDF Url : ADA321239

Report Date : 22 NOV 1996

Pagination or Media Count : 19

Abstract : Several achievements are made through this initial collaborative project These are: (1) creation of enormous capability at this HBcU in enhancing this research; (2) extensive studies on identification of TNT biotransformation products in several bacterial species and one fungal species; (3) identification of several genes which are capable of catalyzing degradation of metabolites of ThT in several microbial species, particularly in the flingus P. chrysosporiwn; (4) isolation and DNA sequence of these genes for probe development; and (5) PCR amplification of 2,4-DNT gene using P. chrysosporiwn genomic DNA as template; (6) DNA:DNA hybridization, (32)P labeled 2,4-DNT dioxygenase gene (used as probe) to anneal with P. chrysosporiwn genomic DNAs; and (7) selection of proper combination of mixed genotypes of bacteria for very efficient degradation of TNT.

Descriptors :   *BACTERIA, *FUNGI, *CONTAMINATION, *TNT, *PSEUDOMONAS, PROBES, DEGRADATION, ISOLATION, EFFICIENCY, DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, SEQUENCES, GENES, METABOLITES.

Subject Categories : Anatomy and Physiology
      Ammunition and Explosives
      Environmental Health and Safety

Distribution Statement : APPROVED FOR PUBLIC RELEASE