Accession Number : ADA321239
Title : Search for Genes Having TNT Degrading Capability for Bioremediation.
Descriptive Note : Final rept. 25 Oct 93-18 Sep 96,
Corporate Author : HOWARD UNIV WASHINGTON DC
Personal Author(s) : Dutta, S. K.
PDF Url : ADA321239
Report Date : 22 NOV 1996
Pagination or Media Count : 19
Abstract : Several achievements are made through this initial collaborative project These are: (1) creation of enormous capability at this HBcU in enhancing this research; (2) extensive studies on identification of TNT biotransformation products in several bacterial species and one fungal species; (3) identification of several genes which are capable of catalyzing degradation of metabolites of ThT in several microbial species, particularly in the flingus P. chrysosporiwn; (4) isolation and DNA sequence of these genes for probe development; and (5) PCR amplification of 2,4-DNT gene using P. chrysosporiwn genomic DNA as template; (6) DNA:DNA hybridization, (32)P labeled 2,4-DNT dioxygenase gene (used as probe) to anneal with P. chrysosporiwn genomic DNAs; and (7) selection of proper combination of mixed genotypes of bacteria for very efficient degradation of TNT.
Descriptors : *BACTERIA, *FUNGI, *CONTAMINATION, *TNT, *PSEUDOMONAS, PROBES, DEGRADATION, ISOLATION, EFFICIENCY, DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, SEQUENCES, GENES, METABOLITES.
Subject Categories : Anatomy and Physiology
Ammunition and Explosives
Environmental Health and Safety
Distribution Statement : APPROVED FOR PUBLIC RELEASE