Accession Number : ADA321344

Title :   Effect of HSP27 on Human Breast Tumor Cell Growth and Motility.

Descriptive Note : Annual rept. 1 Sep 95-31 Aug 96,

Corporate Author : NEVADA UNIV RENO

Personal Author(s) : Hickey, Eileen

PDF Url : ADA321344

Report Date : SEP 1996

Pagination or Media Count : 29

Abstract : This award is a Predoctoral Fellowship to support the doctoral training of Donna Egender. The goal of this research is to investigate the effects of the small stress protein, HSP27, on growth and motility characteristics of normal and tumor-derived human mammary cell lines. Our study is based on the hypothesis that HSP27 is a component of a signal transduction pathway that regulates actin microfilament dynamics. We hypothesize that cells overexpressing HSP27 will show increased motility and altered chemotactic properties, in addition to increased resistance to heat killing and certain drugs. Research completed this year includes optimization of transfection of human MCF7 and MDA231 cells, characterization of sensitivity of mammary cell lines to reagents used in selecting stable transfected cell lines, and selection and assay of clonal cell lines. Donna is introducing the use of adenovirus-mediated transfection, for improved efficiency of transfer of genes into breast cell lines. She has also initiated assays of HSP27 promoter function.

Descriptors :   *HUMANS, *PROTEINS, *BREAST CANCER, DYNAMICS, RESISTANCE, STRESS(PHYSIOLOGY), NEOPLASMS, THESES, SIGNALS, FILAMENTS, MOLECULAR BIOLOGY, GENES, TRANSDUCERS, DRUGS, HEAT, CELLS(BIOLOGY), MAMMARY GLANDS, GROWTH(PHYSIOLOGY), CHEMOTAXIS, MUSCLE PROTEINS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE