Accession Number : ADA321394

Title :   Protein Kinase C Processes and Their Relation to Apoptosis in Human Breast Carcinoma Cells.

Descriptive Note : Annual rept. 1 Sep 95-31 Aug 96,

Corporate Author : PITTSBURGH UNIV PA SCHOOL OF MEDICINE

Personal Author(s) : Lazo, John S. ; Rice, Robert L.

PDF Url : ADA321394

Report Date : SEP 1996

Pagination or Media Count : 54

Abstract : It was reported previously that the specific aim of screening agents that would induce apoptosis in the human breast carcinoma cell line MDA-MB-231 was completed, and that calyculin A was the most effective compound tested. Since then Cdc25 phosphatases were reported as possible key oncogenes in human breast carcinoma. In light of our results with calyculin A, it was decided that a more promising focus for the project would be the biochemical basis for the oncogenic actions of Cdc25 phosphatases in human breast carcinoma. At this time we have examined 18 compounds and discovered a Cdc25 phosphatase inhibitor, called 1f, that selectively inhibits Cdc25A,B, and C, has antiproliferative activity against and causes a G1 block in MDA-MB-231 cells. The pure form of this compound and two other compounds which selectively inhibit Cdc25 phosphatases have been made and also demonstrate an equal if not better inhibition of Cdc25 phosphatases as their combinatorial form.

Descriptors :   *HUMANS, *PROTEINS, *PHOSPHORUS TRANSFERASES, *BREAST CANCER, BIOCHEMISTRY, ENZYMES, NEOPLASMS, INHIBITORS, CELLS(BIOLOGY), GENETICS, CANCER, MAMMARY GLANDS, PHOSPHORYLATION, PHOSPHATASES.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE