Accession Number : ADA321595

Title :   Effects of Hydrostatic Pressure on Mammalian Tissue Cells-Disruption of Cytoskeletal Function, Organization, and Regulation.

Descriptive Note : Final rept. 1 Apr 92-31 May 96

Corporate Author : NORTH CAROLINA UNIV AT CHAPEL HILL DEPT OF BIOLOGY

Personal Author(s) : Salmon, Edward D. ; Harris, Albert K.

PDF Url : ADA321595

Report Date : MAY 1996

Pagination or Media Count : 4

Abstract : To investigate the disruption, organization and regulation of the cytoskeleton of mammalian tissue cells by hydrostatic pressure. APPROACH: Novel optical and fixation chambers are being used in combination with immunofluorescence and video microscopy, biochemical and biophysical techniques to examine pressure-induced changes in the structural organization of the cytoskeletal proteins (including tubulin, actin, myosin II, vinculin, talin, vimentin, and cytokeratin) involved in producing changes in cell shape, motility and contractility.

Descriptors :   *FLUORESCENCE, *MICROSCOPY, *VIDEO SIGNALS, *CELLS(BIOLOGY), *HYDROSTATIC PRESSURE, METHODOLOGY, FIBERS, ORGANIZATIONS, MAMMALS, STRUCTURAL PROPERTIES, BIOCHEMISTRY, PROTEINS, CELL STRUCTURE, BIOPHYSICS, MUSCLE PROTEINS.

Subject Categories : Biochemistry
      Medicine and Medical Research
      Fluid Mechanics

Distribution Statement : APPROVED FOR PUBLIC RELEASE