Accession Number : ADA321820

Title :   Breast Tissue Dosimetry of PhIP (2-amino-1-methyl-6 phenylimidazo (4, 5b) pyridine) at Human-Relevant Exposures.

Descriptive Note : Annual rept. 1 Oct 95-30 Sep 96,

Corporate Author : DEPARTMENT OF ENERGY OAKLAND CA SAN FRANCISCO OPERATIONS OFFICE

Personal Author(s) : Turteltaub, Kenneth W.

PDF Url : ADA321820

Report Date : OCT 1996

Pagination or Media Count : 28

Abstract : The purpose of this research is to define the molecular events leading to the development of PhIP-induced breast tumors and to assess if PhIP exposure at human dietary levels present a human breast cancer risk. Our goals are to understand the effects of chemical exposure on adduct formation and metabolism, the types of adduct formed, how adducts are repaired and the ability of the breast to metabolize PhIP at exposure levels expected to occur via the human diet. To date, we have studied the pharmacokinetics of PhIP in both chronic feeding experiments and acute oral administrations of PhIP. We have continued studies to determine if PhIP is present in the breast tissue of lactating rats and if PhIP is passed from the milk to suckling pups. Additionally, we have investigated the effect of chlorophyllin treatment on the distribution of 14C-PhIP. We are in the process of characterizing the DNA adducts formed by the PhIP and are determining the effect of dose of PhIP on PhIP-DNA adduct formation in female rodents. We have now synthesized PhIP-DNA for antibody production. Furthermore, tritium AMS methodology has now been developed to the point that a double-labeling experiment can now be performed utilizing 2 different compounds.

Descriptors :   *TISSUES(BIOLOGY), *DOSIMETRY, *BREAST CANCER, LABORATORY TESTS, RATS, METHYL RADICALS, HUMANS, CHEMICALS, MOLECULES, SYNTHESIS(CHEMISTRY), METABOLISM, DEOXYRIBONUCLEIC ACIDS, ANTIBODIES, EXPOSURE(PHYSIOLOGY), PHARMACOKINETICS, RODENTS, FEEDING, DIET, FEMALES, PYRIDINES, RADIOACTIVE ISOTOPES, RADIATION DOSAGE, ORAL DOSAGE, MAMMARY GLANDS, TRITIUM, LACTATES, MILK, CHLOROPHYLLS.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research
      Radiobiology
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE