Accession Number : ADA321906

Title :   A Genetic Approach to Identifying Signal Transduction Mechanisms Initiated by Receptors for TGF-B-Related Factors.

Descriptive Note : Annual rept. 1 Oct 95-30 Sep 96,

Corporate Author : WISCONSIN UNIV-MADISON

Personal Author(s) : Hoffman, F. M.

PDF Url : ADA321906

Report Date : OCT 1996

Pagination or Media Count : 7

Abstract : A genetic screen for new mutations affecting the signaling pathway by which cells respond to TGF-beta-related factors in Drosophila was carried out. The genetic background was sensitized using a mutation in the Type I receptor gene. New mutations affecting the phenotypes caused by the sensitized genetic backgrounds were recovered. The new mutations were mapped to six different genes. Three of these genes were previously shown to participate in the signaling pathway, confirming that the sensitized genetic background was appropriate. DNA sequencing is being used to confirm the generation of new mutant alleles in the Mad gene and the Type II receptor gene. Three of the genes in which new mutations were recovered map to regions not previously known to be involved in dpp signaling. Detailed genetic mapping has provided cloning strategies for the molecular identification of two of these genes.

Descriptors :   *GENETICS, *GROWTH(PHYSIOLOGY), *DROSOPHILA, *BREAST CANCER, STRATEGY, MOLECULES, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, REGIONS, IDENTIFICATION, BACKGROUND, GENES, CLONES, GENETIC ENGINEERING, MEDICAL RESEARCH, SENSE ORGANS, GENETIC MAPPING, SENSITIZING.

Subject Categories : Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE