Accession Number : ADA322222

Title :   In Vivo and In Vitro Analysis of the Regulation of C-myc Proto-oncogene Expression in Human Breast Cancer.

Descriptive Note : Final rept. 15 Sep 94-14 Sep 96,

Corporate Author : MASSACHUSETTS GENERAL HOSPITAL BOSTON

Personal Author(s) : Imbalzano, Anthony N.

PDF Url : ADA322222

Report Date : OCT 1996

Pagination or Media Count : 72

Abstract : The purpose of this research was to characterize the factors controlling the expression of c-myc and other genes that are commonly mis-regulated in transformed cells. Genomic DNA exists in the form of chromatin, which generally renders the DNA inaccessible to factors that promote gene expression. Here we report characterization of a cellular protein complex, hSWI/SNF, that can alter chromatin structure in an energy dependent manner and can facilitate the interaction of transcription factors with chromatin. The results indicate that the change in chromatin structure induced by hSWI/SNF is stable in the absence of continued ATP hydrolysis. The increased ability of transcription factors to bind to chromatin is not dependent on concurrent hSWI/SNF activity.

Descriptors :   *GENES, *BREAST CANCER, HUMANS, ENZYMES, PROTEINS, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, HYDROLYSIS, CELLS(BIOLOGY), MAMMARY GLANDS, GROWTH(PHYSIOLOGY), CHROMATIN.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE